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At this point, you might wonder what phenotypes fungi such as yeast and Neurospora express In general, microorganisms have phenotypes that fall into three broad categories: colony morphology, drug resistance, and nutritional requirements Many microorganisms can be cultured in petri plates or test tubes that contain a supporting medium such as agar, to which various substances can be added ( g 615) Wild-type Neurospora, the familiar pink bread mold, generally grows in a filamentous form, whereas yeast tends to form colonies Various mutations exist that change colony morphology In yeast, the ade gene causes the colonies to be red In Neurospora, fluffy ( fl ), tuft (tu), dirty (dir), and colonial (col4 ) are all mutants of the basic growth form In addition, wild-type Neurospora is sensitive to the sulfa drug sulfonamide, whereas one of its mutants (Sfo) actually requires sulfonamide in order to survive and grow.

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Notice that in this new sample (see Listing 9-5), only the Language attribute is specified in the first line Also note that a <script runat="server"> tag is included for you as a place for your server-side VB NET and C# code Listing 9-5 Using the Single-File Option <%@ Page Language="C#" %> <!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 11//EN" "http://wwww3org/TR/xhtml11/DTD/xhtml11dtd"> <script runat="server"> protected void Button1_Click(object sender, EventArgs e) { //add event handling code here } </script> <html xmlns="http://wwww3org/1999/xhtml" > <head runat="server"> <title>Untitled Page</title> </head> <body> <form id="form1" runat="server"> <div> <asp:Button ID="Button1" runat="server" OnClick="Button1_Click" Text="Button" /> </div> </form> </body> </html> As in Listing 9-4, we added a button to the web form We did this to highlight one of the major differences between the code-behind and the single-file options.

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Yeast shows similar sensitivities to antifungal agents Nutritional-requirement phenotypes provide great insight not only into genetic analysis but also into the biochemical pathways of metabolism, as mentioned in chapter 2 Wild-type Neurospora can grow on a medium containing only sugar, a nitrogen source, some organic acids and salts, and the vitamin biotin This is referred to as minimal medium However, several different mutant types, or strains, of Neurospora cannot grow on this minimal medium until some essential nutrient is added For example, one mutant strain will not grow on minimal medium, but will grow if one of the amino acids, arginine, is added ( g 616) From this we can infer that the wild-type has a normal, functional enzyme in the synthetic pathway of arginine.

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The arginine-requiring mutant has an allele that speci es an enzyme that is incapable of converting one of the intermediates in the pathway directly into arginine or into one of the precursors to arginine We can see that if the synthetic pathway is long, many different loci may have alleles that cause the strain to require arginine ( g 617) This, in fact, happens, and the different loci are usually named arg1, arg2 , and so on There are numerous biosynthetic pathways in yeast and Neurospora, and mutants exhibit many different nutritional requirements Mutants can be induced experimentally by radiation or by chemicals and other treatments These, then, are the tools we use to analyze and map the chromosomes of microorganisms, including yeast and Neurospora These techniques are expanded on in the next chapter..

In addition to needing to know the location of an object, as in the previous pair of plug-ins, you often need to know their width and height, which you can determine with these functions Figure 3-11 shows the plug-ins being used to discover an object s width and height

Since Listing 9-4 used the code-behind option, code handling for the button s Click event will be within the Defaultaspxcs file However, looking at the single-file example (Listing 9-5), you can see that the code for the Click event now goes within the server-side script block In summary, when you use the code-behind option, you place event handling code in a separate code file not in the aspx page This file will be named whatever you named the aspx file, but with a vb or cs extension added on at the end..

Individual spores are pressed out of the ascus. The spores are lined up and the agar is sliced into sections.

Unordered Spores (Yeast)

Baker s, or budding, yeast, Saccharomyces cerevisiae, exists in both a haploid and diploid form ( g. 6.18). The